Laboratory of genetics

Laboratory of genetics

	Saenko Artem Mykhailovych c. at. head of the laboratory candidate rural-urban Sciences, senior researcher This email address is being protected from spambots. You need JavaScript enabled to view it.
	Buslyk Tetyana Volodymyrivna senior researcher candidate rural-urban of Sciences, senior researcher -->
	Pochernyaev Konstantin Fedorovich chief researcher Dr. S.-G. of Sciences, senior researcher This email address is being protected from spambots. You need JavaScript enabled to view it.
	Pochernyaeva Elizaveta Aleksandrovna junior researcher This email address is being protected from spambots. You need JavaScript enabled to view it.
	Peka Mykyta Yuriyovych researcher This email address is being protected from spambots. You need JavaScript enabled to view it.

Scientific research

PND 31. "Genetic improvement of farm animals, their reproduction and preservation of biodiversity"

("Genetics, preservation and reproduction of bioresources in animal husbandry")

Task 31.01.00.06.F. "To investigate the relationship between telomere length and SNP genes of the telomerase complex with productive traits of pigs raised under intensive technologies" (2021-2025, state registration number 0121U109836). (Head – Balatsky V. M.; performers Balatsky V. M., Saenko A. M., Korinny S. M., Budakva E. O., Peka M. Yu., Ilenko M. O.; 2021-2025)

Task 31.01.00.07.F. "To study the pleiotropic effect of genes, SNPs of which are used in marker-associated breeding of pigs" (2021-2025, state registration number 0121U109838) (Head – Pochernyaev K.F.; executors Povod M. G., Balatsky V. M., Mazur O. O., Budakva E. O., Matiyuk V. V.; 2021-2025)

Areas of scientific activity

Development of methods for determining DNA markers.
Establishment of associations of DNA markers with productive traits of pigs and meat quality.
Control and monitoring of the breeding process on based on DNA typing of animals using SNP and STR markers.
Determination of the origin of animals and the purity of genealogical structures by analyzing the polymorphism of microsatellite loci and mitochondrial DNA of pigs.
Determination of the content of genetically modified organisms (GMOs) in plant raw materials, feed and consumer products

Main achievements of the genetics laboratory

development and implementation of methods for assessing pig genotypes using DNA markers for marker-based breeding in pig breeding. In particular, a DNA test for stress sensitivity in pigs, a DNA test for multiparity in sows, and genotyping methods for genes responsible for the manifestation of meat and fattening traits in pigs have been developed.
The relationships of a number of loci of quantitative traits with meat qualities and meat quality indicators of large white pigs for marker selection have been established.
A genotyping technique for microsatellite loci of the pig genome has been developed, including multiplex systems for determining the origin of animals and conducting genetic and population studies.
A haplotyping method for six loci of the pig mitochondrial genome has been developed to determine maternal purebredness, homogeneity of genealogical and factory families, conduct genetic and population studies, and archaeogenetic studies.
Study using various types of molecular markers, the genetic structure of a number of domestic and foreign breeds, types and lines of pigs, monitoring of genetic processes in pig populations is carried out to assess and adjust breeding measures.
a system of early complex molecular genetic assessment of boars for breeding farms, stud farms and artificial insemination stations with high potential for reproductive and meat qualities has been developed
studies are being conducted to analyze the distribution of gene mutations in pig populations.

Object of research and implementation

Patents and copyrights

Patent of Ukraine for utility model No. 65963 MPK A01K 67/00. Method of genotyping pigs for the growth hormone gene by BsuRI-polymorphic restriction site / V.M. Balatsky, K.F. Pochernyaev, A.M. Saenko, applicant and patent owner Institute of Pig Breeding named after O.V. Kvasnytsky NAAS of Ukraine. u 2011 03989; filed 04.04.2011; published 26.12.2011, Bull. No. 24.
Ukrainian Patent for Invention No. 114145 MPK A01K67/02. Method for Genotyping Pigs Based on Microsatellite Markers with Tetranucleotide Repeats / S. M. Korinny, K. F. Pochernyaev, Applicant and Patent Owner Korinny Sergey Nikolaevich. A 2015 12620; filed 12/21/2015; published 04/25/2017, Bull. No. 8.
Ukrainian Patent for Utility Model No. 141125 MPK C12Q 1/6806; C12Q 1/6876; C12Q 1/6888. Method for identifying a DNA fragment of Candidatus Clavochlamydia Salmonicola bacteria by amplifying a section of the 16S rRNA gene in polymerase chain reaction / V. K. Zezekalo, K. F. Pochernyaev, applicant and patent owner Institute of Pig Breeding and Agroindustrial Production of the NAAS. u 2019 08681; filed on 07/18/2019; published on 03/25/2020, Bull. No. 6.
Patent of Ukraine for a utility model No. 117492 MPK .A61K 39/118. Method for identifying Chlamydia Pneumoniae DNA in polymerase chain reaction by amplification of a fragment of the major membrane protein (MOMP) for its species differentiation / M. V. Kornienko, I. M. Ksyonz, K. F. Pochernyaev, applicant and patent owner Institute of Pig Breeding and Agroindustrial Production of the NAAS. u 2017 00832; filed 01/30/2017; published 06/26/2017, Bull. No. 12.
Certificate of conformity of the state of the measurement system

Main publications
1. Balatsky, V., Oliinychenko Y. K., Buslyk T. V., Bankovska I. B., Saienko A., Korinnyi S., Pocherniaev K. Associations of QTL Region Genes of Chromosome 2 with Meat Quality Traits and Productivity of the Ukrainian Large White Pig Breed// Cytology and Genetics. 2021 - Vol. 55, No. 1, pp. 53–62. https://doi.org/10.3103/S0095452721010023
2. Balatsky, V., Oliinychenko Y. K., Buslyk T. V., Bankovska I. B., Saienko A., Korinnyi S., Pocherniaev K. Associations of QTL Region Genes of Chromosome 2 with Meat Quality Traits and Productivity of the Ukrainian Large White Pig Breed// Cytology and Genetics. 2021 - Vol. 55, No. 1, pp. 53–62. https://doi.org/10.3103/S0095452721010023
3. Bankovska, Y. Oliinychenko, V. Balatsky, T. Buslyk, S. Hryshchenko, R. Susol (2020). Association of LEP-and CTSF-genotypes with levels of meat quality PSE, NOR and DFD in pigs of large white breed of Ukrainian selection. Agricultural Science and Practice, 7(1), 14-23. https://doi.org/10.15407/agrisp7.01.014
4. P. A. Vashchenko, V. M. Balatsky, K. F. Pocherniaev, V. M. Voloshchuk, V. H. Tsybenko, A. M. Saenko, Ye. K. Oliynychenko, T. V. Buslyk, H. S. Rudoman. (2019). Genetic characterization of the Mirgorod pig breed, obtained by analysis of single nucleotide polymorphisms of genes. Agricultural Science and Practice, 6(2), 47-57. https://doi.org/10.15407/agrisp6.02.047
5. Zezekalo V.K., Peredera S.B., Buslyk T.V., Pochernyaev K.F., Shcherbakova N.S. (2019). PCR test systems for the detection of Clavichlamydia salmonicola and Piscichlamydia salmonis in fish. Guidelines for the preparation of an article submitted for publication in the "Journal of Veterinary Medicine, Biotechnology and Biosafety", 20.
6. V. Balatsky, Y. Oliinychenko, N. Sarantseva, A. Getya, A. Saienko, V. Vovk, O. Doran (2018). Association of single nucleotide polymorphisms in leptin (LEP) and leptin receptor (LEPR) genes with backfat thickness and daily weight gain in Ukrainian Large White pigs. Livestock Science, 217, 157-161. https://doi.org/10.1016/j.livsci.2018.09.015
7. Balatsky V.N., Pochernyaev K.F., Buslyk T.V., Dykan O.S., Korinnyi S.N., Pena R., Doran O. (2015) Sequence variation in the cathepsin B (CTSB), L (CTSL), S (CTSS) and K (CTSK) genes in Ukrainian pig breeds. Global J. Anim. Breed. Genet., 3 (3), 117-124.
8. Kubejko J, Clop A, Balatsky V, Pochernyaev K, Eghbalsaied S, Amills M (2017). Mitochondrial DNA variation in Ukrainian wild boars. Animal Genetics, 48(6), 725-726. https://doi.org/10.1111/age.12592
9. Korinny S. M. (2015). Microsatellite DNA loci panel with tetranucleotide motif. Ukrainian Animal Husbandry, (8), 12-15.
10. Balatsky V., Saranceva N., Oliynychenko E. et al. (2017). Polymorphism of leptin (LEP) and leptin receptor (LEPR) genes and their association with meat and back fat quality in Ukrainian Large White pig breed. Proceedings of CRIB Annual Meeting 2017. Centre for Research in Bioscience, University of the West of England. 13 January 2017, p. 25.
11. Viktor Balatsky, Irina Bankovska, Ramona N. Pena, et al. (2016). Polymorphisms of the porcine cathepsins, growth hormone-releasing hormone and leptin receptor genes and their association with meat quality traits in Ukrainian Large White breed. Molecular Biology Reports, 43, 517–526. https://doi.org/10.1007/s11033-016-3977-z
12. Rudoman G.S., Balatsky V.N. (2014). Genetic structure of Large White pigs of Ukrainian and English selection on the FUT1 and MUC4 genes associated with animal resistance to colibacteriosis. Pig Breeding, 65, 154-160. (in Ukrainian, English abstract).
13. Pochernyaev K. F., Lomako D. V. (2012) Genetic diversity of mitochondrial genomes of large black sows. Pig breeding, No. 61., 46-51.
14. Balatsky V. N., Saenko A. M., Gryshina L. P. (2012) Polymorphism of estrogen1 receptor locus in populations of pigs of different genotypes and its association with reproductivity traits of Large White sow. Cytology and Genetics, 46(4), 233-237.
15. Balatsky V. Bankovska I., Buslyk T., et al. (2012). Genetic markers and the quality of meat of the Ukrainian local breeds of pigs. Excelmeat- Workshop, Lleida, Spain, P. 16.
16. Korinny, S. M. (2008). Population genetic characteristics of the large white breed of pigs using microsatellite markers. Scientific Bulletin of the Lviv National University of Veterinary Medicine and Biotechnology named after S.Z. Gzhytsky, 10(3-3) (38).
17. Korinnyi, S. M., Pochernyaev, K. F., & Balatskyi, V. M. (2005). Animal wool as a convenient object for DNA isolation for PCR analysis. Veterinary biotechnology: Bull. IVM UAAN, (7), 80-83.

Material and technical base of the genetics laboratory

Vertical electrophoresis gel documentation system

DNA isolation zone Amplification zone Electrophoresis

Separation of protein mixture in a Vortex Device for rapid vertical electrophoresis device mixing solutions in test tubes up to 50 ml.

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